I put a 2nd. Gen Colt 32-20 barrel & cylinder in a Uberti .45 Colt several years ago.
The cylinder dropped in and timed perfectly, with a new base pin bushing fit to the frame.
But the Colt barrel had to be shortened on a lathe, and a new forcing cone cut to fit the cylinder.
I would expect tolerances are held close enough they don't cut every barrel for every frame and cylinder now though.
They ain't got time for that and still make a profit.
I would expect the chances are good a second Uberti cylinder would drop right in.
But, I could be wrong too??
You pays your money, and you takes your chances.
Unless you send it back for a new cylinder, or have the means to correct it yourself.
rc