Murf: I do believe that powder placement does make a difference, and I believe that what we see depends on the powder and the quantity. But there is something that I don't see in your data, and is a sufficient number of shots to calculate averages with any confidence. .
I think Blue Dot is not a good 45 LC powder based on my data. All of my charges are thrown on a Dillion 550B and you can see how much tighter the ES's and SD's are for Unique compared to Blue Dot. Blue Dot has extreme spreads of hundreds of feet per second. That is just awful in my opinion, and if your data strings are small, you may not realize that velocity averages are going to be highly variable just due to the powder and that might be masking velocity changes due to position.
I don't recommend Blue Dot for the 45 LC because of these high extreme spreads. I don't have the means to measure pressure curves, or see pressure curves, all I can see is velocity. But, I am a worry wart and I want as low of SD's and ES's as possible, because I believe that consistency is good, and consistency provides safety. I could be wrong, but someone better provide pressure curve data from tests, before I will believe them. I do know that Hercules dropped 45 LC loads and Blue Dot but Alliant has Blue Dot for the 250 LSWC but not 250 JHP. I don't think that is particularly encouraging.
5 1/2" USFA Rodeo
250 LRN (.452") 8.5 grs Unique thrown, R-P cases, CCI300 primers
12-May-02 T = 80°F
Ave Vel =855
Std Dev =16
ES 59.91
High 885.4
Low 825.5
Number Shots 12
250 LRN (.452") 11.5 grs Blue Dot R-P cases, WLP
11-Sep-05 T = 85 °F shoots close to point of aim acceptable accuracy
Ave Vel =845
Std Dev =48
ES 183.6
High 933.3
Low 749.7
N = 18
250 LRN (.452") 12.0 grs Blue Dot R-P cases, WLP
11-Sep-05 T = 85 °F shoots close to point of aim good accuracy
Ave Vel =904
Std Dev =44
ES 149.6
High 955.2
Low 806
N = 15
I will agree that 12 shots is not a large sample size, and if I search my data, I am certain that I have some 32 shot strings with Unique, but not with the same 45 LC which I shot Blue Dot. I did not shoot large quantities of Blue Dot in 45 LC over the chronograph, so my sample sizes are also small. But, I shot enough to convince myself that Blue Dot and the 45LC is not a good combination.
This is Unique data in a different pistol:
M625-9 Mountain Gun 4" Barrel
250 LRN(.452")Tenn Valley Bullet 8.5 grs Unique lot UN331 1989 , R-P cases WLP (brass)
16-May-09 high 83 °F
Ave Vel = 908
Std Dev = 23.3
ES = 92.5
High = 965.3
Low = 872.8
N = 29