For general info, I'll also post this here:
The first step is to insure the problem isn't grit or fouling under the ejector.
This is THE most common cause of binding Colt cylinders, can "come and go" and can happen fairly often.
Press the ejector rod and hold it in while you use a clean toothbrush to give the underside of the ejector and it's seat in the rear of the cylinder a THOROUGH scrubbing.
Tiny particles of grit and burned powder can get caught under the ejector and become embedded. This causes the ejector to fail to seat fully, which causes the cylinder to bind.
Usually this causes the binding to occur on only one or two chambers, and it tends to come and go.
Since hard grit can really stick to the metal, and sometimes is impossible to see, REALLY SCRUB the ejector and cylinder seat.
If it still binds after a good cleaning, DO NOT disassemble the cylinder for the following reasons:
1. You won't be able to fix a problem.
These things are NOT user repairable and you'll only damage the cylinder assembly trying.
Unless you see an obvious problem like a badly bent ejector rod, there's is NOTHING you can fix, unless you're a fully qualified COLT pistolsmith.
In truth, the usual mechanical problem with a binding cylinder isn't the ejector assembly, it's a bent crane.
2. There are two methods of disassembling the Python, the old method and the new.
The old method REQUIRES a special wrench, and even with it, there is a high risk of ruining the assembly. The ejector tends to cross thread badly, and this destroys the assembly.
If cleaning the cylinder assembly doesn't correct the problem, I strongly recommend sending the gun to the Colt factory or Pittsburgh Handgun Headquarters for a professional repair.
No BS, these cylinder assemblies are EASY to screw up, terribly expensive to replace, and completely non-user repairable.
If this was an older Colt that was well worn, and not worth much you might go for it, but that's not smart on a super expensive Python.
I'm telling you this because I'm a retired gunsmith specializing in Colt revolvers, and I've seen WAY too many good guns damaged this way.
Again, unless it's grit or dirt under the ejector there is NOTHING at all you can do to correct it, and trying is guaranteed to ruin the assembly.
