You really cannot machine a cylinder to shoot 45 Colt, 45 ACP and 45 Auto Rim. Typically you only get two of the three, 45 ACP and 45 Auto-Rim OR 45 Colt and 45 ACP. You typically machine a cylinder differently if the primary cartridge is rimmed vs rimless.
With a rimmed cartridge like 45 Colt you typically leave a rim around the outside edge of the cylinder. This raise rim allows the rimmed cartridge to head space with or with out a moonclip present. The picture below is a 357 Magnum but it is cut for moonclips and you can see the raised rim around the outside of the cylinder, thus allowing un-moonclipped cartridges to headspace correctly. So with a 45 Colt you would machine the cylinder similarly allowing 45 Colt to work with or without the moonclip and 45 ACP only with a moonclip. 45 Auto-Rim would not work as 45 AR's rim is so thick that it would not allow the cylinder to close.
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357 Magnum cylinder cut for moonclips, but no moonclip present
With a rimless cartridge like a S&W 625 chambered in 45 ACP the cylinder is typical machined flat with no raise rim around the outside of the cylinder since there is no rim to use it to head space with 45 ACP. This face is machined flat for ease of manufacture and so that a 45 ACP with a moonclip head spaces correctly and thus 45 Auto Rim must have a rim as thick as both a 45 ACP rim and the moonclip. If the chambers were lengthened to 45 Colt length the cartridge would have nothing to head space against as face of the cylinder is be too far forward for the 45 Colt Rims to head space against. Even if you put the 45 Colt on moonclips they would likely not head space correctly due to how much thinner 45 Colt moonclips are then 45 ACP moonclips.
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45 ACP without a moonclip.
The ring you might see around my cylinder her is not raised that is simply were the edge of the moonclips has burnished the stainless there. The face of this cylinder is flat.
Personally I would cut the 25 to use moonclips. You can still use 45 Colt with or without moonclips and 45 ACP on moonclips is one of the true joys of owning a revolver. Moonclips rule!