You will have to try it and see. My guess is that the rim will likely be too thick to permit you to close the cylinder. But, most probably, you can use the .45 S&W (Schofield) round although the rim is a smidgen wider than the .45 Colt rim. If you reload, Starline is making a .45 Colt case sized down to the height of the .45 acp. It is essentially an auto rim case with out the thick rim. You can load this round using .45 acp dies with a .45 Colt shell plate.