To a certain extent, yes. But most shooters will not be good enough to actually be able to detect the difference between a bullet that has to jump a bit of freebore, compared to one that enters the chamber throat without any freebore. In theory, a bullet leaping across a chamber without any support for 1/10" or so could acquire some wobble, which might affect accuracy as it gets forced into the chamber throat and then the forcing cone. But in actuality, one would have to be a very good shooter to detect this.
Near the end of the 19th Century Smith and Wesson introduced a couple of special target cartridges called the 38-44, and the 32-44. Not to be confused with the 38-44 cartridge of the 1930s, this 38-44 was basically an extra long 38S&W, that extended the full length of the cylinder. Same with the 32-44. The '44' part of the cartridge name simply denoted that these cartridges were used in the large frame Top Break revolvers that were usually chambered for 44 caliber cartridges like the 44 Russian. The bullet was seated down inside the case, sub-flush. The idea was there was no free bore, and there was no chamber throat. The bullet left the confines of the cartridge case and entered the forcing cone directly, with no opportunity to wobble. Target accuracy records were achieved with these cartridges that have never been equaled, but this was at the international Olympic level of competition.
For most of us, a little bit of freebore does not matter much.
