I have an "old model" Blackhawk convertible in .45 caliber (Colt or Auto). The .45 Auto's case mouth butts up against a shoulder in the chamber, just as it does in the chamber of an autoloading pistol's barrel. Moon clips are only needed to extract the cases from a double-action revolver, giving the extractor star something to push against; they do not affect loading or (in most cases) firing. A single-action revolver has an ejector rod that goes through the center of the chamber hole and pushes against the case head (if fired) or bullet of the round in the chamber.
Although I have only put a box apiece of .45 Colt and Auto cartridges through mine, hand held, it seems to be equally accurate with either cartridge. However, contemporary Colt and Auto bullets are both the same diameter.