Several characters in Dexter have revolvers. The two that come to mind are Masuka with his ginormous piece at the bowling alley and Boyd Fowler from Season 5.
In Flashpoint (the TV show, not the movie), I've seen them use revolvers with their shield. There are a few episodes where bad guys have revolvers and the cops are counting down to when they're empty. The Judge is also used by a drug dealer in Season 1 (called "The Jury" on the show - they seem to purposefully misname guns on that show for some reason). They also use rubber bullets in revolvers.
One of the most powerful guns in Half Life is the Colt Anaconda.
In Firefly, they have what look like old style guns (lever actions and revolvers) that have futuristic sounds when being charged.
The tow truck driver from Men in Black carried a big wheelgun.
In the first season of Battlestar Galactica (the new one), they had a heavily modified 686. Later they used a virtually unmodified fiveseven.
There are also a few 4-shot derringers in BG.
In Psych, there is an episode where Lassider gets in a Western shootout with the bad guy, where the bad guy has a revolver and Lassider has his Glock. Lassider also owns a 4-shot derringer which appears to be his bump-in-the-night weapon.
On Police Academy, the big redneck brought a giant pistol to the practice range. When the instructor asked where he got it, he responded "My Mama got that for me!"
Stallone's character in The Expendables uses a single action revolver. Jason Statham is more of a ludite, he uses knives.